FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_91.3510000005e1d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_91.3510000005e1d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6713
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA841.251303441084463No Hit
CTAGCTGGATGTCTGTCTCTTATACAC300.4468940861015939No Hit
CTAGCTGGATGTCTGTCTCTTCTACAC140.20855057351407716No Hit
CTAGCTGGATGCTGTCTCTTATACCCA120.17875763444063758No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT120.17875763444063758No Hit
CTAGCTGGATGCTGTCTCTTCTACACA120.17875763444063758No Hit
CTAGCTGGATCTGTCTCTTATCCACAT110.16386116490391778No Hit
CTAGCTGGATGCTGTCTCTTATACCCC90.13406822583047817No Hit
CTAGCTGGATCTGTCTCTTATACACAT90.13406822583047817No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA90.13406822583047817No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA80.11917175629375838No Hit
CTAGCTGGATGTAGGCTGTCTCTTATA70.10427528675703858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTG2800.021.0000027
GATGTTA353.788968E-521.0000028
GATGTCG555.657057E-921.0000028
GATGTAG555.657057E-921.0000028
ATGTGGA353.788968E-521.0000029
GCGTTTT353.788968E-521.00000218
ATTTTTT1100.021.00000220
GGATGTT1350.021.07
GGATGTC1050.021.07
GGATGTA1200.021.07
GATGTTG606.184564E-1021.08
TGTTTTT454.665544E-721.019
GATGTGC606.184564E-1021.08
GATGTGA454.665544E-721.08
GATGTCA250.003046094421.08
GATGTAT303.400334E-421.08
GTCTGTC454.665544E-721.020
ACTTTTT303.400334E-421.019
ATGTGGT454.665544E-721.09
ATGTGCT250.003046094421.09
Produced by FastQC (version 0.11.2)