FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_90.3510000005e1ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_90.3510000005e1ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTC250.003098957721.09
ACTGCGC250.003098957721.09
GGGTTTT454.916692E-721.018
ACTGAGG250.003098957721.09
ACTGAGA250.003098957721.09
TTCTGTC303.4835204E-421.021
GGATTTT250.003098957721.018
CTCTTAT250.003098957721.021
CCAGAGA7200.021.03
GGTTTTT900.021.019
GTCTGTC353.9135462E-521.020
CATTTTT505.4911652E-821.019
GAGTTTT250.003098957721.018
TCCAGAG6750.021.02
GCCAGAG250.003098957721.02
TGCCAGA250.003098957721.01
CCCGAGA505.4911652E-821.03
TATTTTT454.916692E-721.019
GATTTTT505.4911652E-821.019
GACTGTT454.916692E-721.08
Produced by FastQC (version 0.11.2)