FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_9.3510000005d7ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_9.3510000005d7ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50603
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA3900.7707052941525205No Hit
TACGGTACGTAGCCTGTCTCTTATACA1890.3734956425508369No Hit
TACGGTACGTAGTCTGTCTCTTATACA1310.25887793213841076No Hit
TACGGTACGTAGACTGTCTCTTATACA1110.21935458372033279No Hit
TACGGTACGTAGCTGTCTCTTATACAC960.1897120724067743No Hit
TACGGTACGTAGGGCTGTCTCTTATAC900.17785506788135091No Hit
TACGGTACGTAGGGGCTGTCTCTTATA660.13042704977965733No Hit
TACGGTACGTAGAGCTGTCTCTTATAC510.10078453846609885No Hit
TACGGTACGTAGCTGTCTCTTCTACAC510.10078453846609885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA303.8378825E-421.0197831
TTCGGTA950.021.0197831
TATGGTA1200.021.0197831
TACGGTA47300.021.0197811
GCTTTTT2350.020.99901419
GTAGATG751.8189894E-1220.9990129
TAGAAGT250.00333683620.99901210
TTGTTTT800.020.99901218
TAGAACA303.863663E-420.99901210
TAGCGGG456.143582E-720.99901210
GTAGAGC651.1823431E-1020.9990129
GTAGAGA900.020.9990129
TGCATTT250.00333683620.99901217
GTAGACA601.0004442E-920.9990129
GTAGAAG651.1823431E-1020.9990129
GTAGAAC507.2130206E-820.9990129
GTAGAAA405.246058E-620.9990129
GCCTTTT601.0004442E-920.99901218
CAGTTTT800.020.99901218
ATCTTTT456.143582E-720.99901218
Produced by FastQC (version 0.11.2)