FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_87.3510000005e16a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_87.3510000005e16a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5331
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA130.24385668730069407No Hit
ACTTCGTCAGCTGTCTCTTATACACAT110.20634027386981804No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC90.16882386043894204No Hit
ACTTCGTCAGCTGTCTCTTATCCACAT70.13130744700806604No Hit
ACTTCGTCAGCTGGGGGGGGTTTTTTT60.11254924029262803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCA303.2683247E-421.0000028
CAGCTAT303.2683247E-421.0000028
CAGCTAC454.2801003E-721.0000028
ACGTCGT303.2683247E-421.0000021
GTCGTCA303.2683247E-421.0000023
TCAGCTT1200.021.0000027
TATTTTT303.2683247E-421.00000219
CGTCGTC303.2683247E-421.0000022
GCTTTTT353.593153E-521.00000219
CAGCTTT303.2683247E-421.0000028
CAGCTTC303.2683247E-421.0000028
CAGCTGC303.2683247E-421.0000028
CAGCTCT250.002961536921.08
CAGCTCG403.932131E-621.08
CAGCTAG403.932131E-621.08
CGTCAGC4950.021.05
TTCGTCA4150.021.03
AGCTACG250.002961536921.09
TTTTTTT3700.021.021
TGTTTTT403.932131E-621.019
Produced by FastQC (version 0.11.2)