FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_86.3510000005e141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_86.3510000005e141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA390.575390970787843No Hit
TGTTGCCGAACAGCTGTCTCTTATACA160.23605783416937148No Hit
TGTTGCCGAACATCTGTCTCTTATACA90.13278253172027146No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT90.13278253172027146No Hit
TGTTGCCGAACACCTGTCTCTTATACA80.11802891708468574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCC6000.021.0000021
GGGTTTT505.163929E-821.00000218
AACAAGG250.00304848921.0000029
ACATGGC250.00304848921.00000210
AATTTTT505.163929E-821.00000219
GGATTTT250.00304848921.00000218
ACTTTTT250.00304848921.00000219
CGAACAG2000.021.0000027
GATTTTT505.163929E-821.00000219
GAACATG750.021.0000028
GAACAGA250.00304848921.0000028
GAACACT250.00304848921.0000028
GAACAAG750.021.0000028
AACAGGA250.00304848921.0000029
GCTTTTT750.021.00000219
ATTTTTT1500.021.00000220
AGTTTTT555.677066E-921.00000219
AACATTG250.00304848921.0000029
GTTGCCG6000.021.0000022
AACACGT250.00304848921.0000029
Produced by FastQC (version 0.11.2)