FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_84.3510000005e10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_84.3510000005e10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA510.5308076602830974No Hit
CAGAACGACAGTGGCTGTCTCTTATAC170.17693588676103247No Hit
CAGAACGACAGTAGCTGTCTCTTATAC100.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT4550.021.00000221
GAACGAC8550.021.0000023
ACGACAG9150.021.0000025
CGACAGT9400.021.06
ACAGTGG1450.021.08
ACAGTGA658.185452E-1121.08
TGGTTTT354.0231098E-521.018
ACAGTCT658.185452E-1121.08
ACAGTCA303.5561965E-421.08
CAGTCGT303.5561965E-421.09
CAGTCGG303.5561965E-421.09
CAGTTGG354.0231098E-521.09
CAGCACG354.0231098E-521.01
GACAGTT1900.021.07
GACAGTG3800.021.07
GACAGTA1700.021.07
AATTTTT303.5561965E-421.019
TTCTGTC303.5561965E-421.021
AGTGGGG303.5561965E-421.010
GTTTTTT2400.021.020
Produced by FastQC (version 0.11.2)