FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_83.3510000005e0e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_83.3510000005e0e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24217
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA1840.7597968369327331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT609.240466E-1021.00000218
ACTGCGG405.059035E-621.00000210
ACTGCGC303.782385E-421.00000210
ACTGCGA303.782385E-421.00000210
ACTGTGG303.782385E-421.00000210
ACTCGGG609.240466E-1021.00000210
ACTGAGG303.782385E-421.00000210
AGGACTT4800.021.0000027
TTTCTGT405.059035E-621.00000221
GGACTCA800.021.0000028
CATTTTT1050.021.00000219
CCGTTTT405.059035E-621.00000218
TAGTTTT303.782385E-421.00000218
TGCTTTT303.782385E-421.00000218
GTCTTTT405.059035E-621.00000218
TATTTTT1200.021.00000219
GACTGTA405.059035E-621.0000029
ATGTTTT405.059035E-621.00000218
GACTGGT609.240466E-1021.0000029
GACTGCT609.240466E-1021.0000029
Produced by FastQC (version 0.11.2)