FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_82.3510000005e0c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_82.3510000005e0c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC271.0522213561964147No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC110.4286827747466875No Hit
ACTCGTCTCGCTGTCTCTTATACACAT80.3117692907248636No Hit
ACTCGTCTCGATGGCTGTCTCTTATAC40.1558846453624318No Hit
ACTCGTCTCGATCCTGTCTCTTATACA30.11691348402182386No Hit
ACTCGTCTCGATGGGCGGGATTTTTTT30.11691348402182386No Hit
ACTCGTCTCGATGGCGGCGGTTTTTTT30.11691348402182386No Hit
ACTCGTCTCGATGCTGTCTCTTATACA30.11691348402182386No Hit
ACTCGTCTCGATGCTGTCTCTTATACC30.11691348402182386No Hit
ACTCGTCTCGATACGGGGGCTTTTTTT30.11691348402182386No Hit
ACTCGTCTCGATCGGTAGGGTTTTTTT30.11691348402182386No Hit
ACGCGTCTCGATCTGTCTCTTATACAC30.11691348402182386No Hit
ACTCGTCTCGATCGGGGGGGTTTTTTT30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG352.7053582E-521.08
TTTTTTT1950.021.021
TGTTTTT352.7053582E-521.019
TCTCGAT2550.021.06
TCGTCTC2050.021.03
TCTTTTT302.6474395E-421.019
ATTTTTT402.719351E-621.020
CGTCTCG2350.021.04
CTCGATT602.3101165E-1021.07
CTTTTTT602.3101165E-1021.020
ACTCGTC2000.020.9999981
TCGATTG250.002551460920.9999988
TCGATGT250.002551460920.9999988
TCGATGA250.002551460920.9999988
GTTTTTT950.020.99999820
GGTTTTT250.002551460920.99999819
GTCTCGA2450.020.9999985
CGATGTG250.002551460920.9999989
CTCGTCT2000.020.9999982
CTCGATG950.020.9999987
Produced by FastQC (version 0.11.2)