FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_80.3510000005e085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_80.3510000005e085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21257
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC870.4092769440654843No Hit
GGATAGTTAGCCTGTCTCTTATACACA390.18346897492590675No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC350.1646516441642753No Hit
GGATAGTTAGCCGGGGGGGGTTTTTTT220.10349531918897303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGTC455.6822137E-721.04481321
GTCTGTC354.264593E-521.04481320
TTTCTGT404.9181253E-621.04481121
GTTTCTG250.003232796721.04481120
ATCTGTC404.9181253E-621.04481120
AGCCCGG900.020.9952969
TTATTTT455.8138176E-720.99529618
GAGTTTT455.8138176E-720.99529618
GCCGGAG455.8138176E-720.99529610
GCCCGGG455.8138176E-720.99529610
AGCCGGC455.8138176E-720.9952969
AGCCGGA900.020.9952969
AGCCGAG455.8138176E-720.9952969
GCTTTTT900.020.99529619
TTAGCCG8850.020.9952967
TTAGCCA3600.020.9952967
CGATTTT455.8138176E-720.99529618
AGCCCGT354.3433884E-520.9952959
AGCCTGG557.821654E-920.9952959
AGCCTGC354.3433884E-520.9952959
Produced by FastQC (version 0.11.2)