FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_79.3510000005e06b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_79.3510000005e06b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12503
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA260.2079500919779253No Hit
CAGACATCGCAATCTGTCTCTTATACA130.10397504598896265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT608.021743E-1021.00000218
CAGTTTT303.64193E-421.00000218
GCAAGTT303.64193E-421.0000029
GCAAGTC303.64193E-421.0000029
GCAAGTA303.64193E-421.0000029
GCAAGGA557.044946E-921.0000029
GCAAGCT303.64193E-421.0000029
GCAAGCA303.64193E-421.0000029
GCAAGAG303.64193E-421.0000029
GTATTTT303.64193E-421.00000218
GTCTGTC303.64193E-421.00000220
CATTTTT303.64193E-421.00000219
GCAATGC303.64193E-421.0000029
GCAATCG303.64193E-421.0000029
GCAATAA303.64193E-421.0000029
GCAAATG303.64193E-421.0000029
TCGCAAG4800.021.0000027
TCGCAAA2200.021.0000027
TGTGTTT303.64193E-421.00000217
CGCAATC608.021743E-1021.0000028
Produced by FastQC (version 0.11.2)