FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_78.3510000005e042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_78.3510000005e042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC320.585865983156353No Hit
AGTGTGACGAACCTGTCTCTTCTACAC250.4577077993409008No Hit
AGTGTGACGAACGGGGGGGGTTTTTTT60.10984987184181619No Hit
AGTGTGACGAACGCTGTCTCTTATACA60.10984987184181619No Hit
AGTGTGACGAACGGCTGTCTCTTATAC60.10984987184181619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTTT555.0622475E-921.00000219
CGAACGC555.0622475E-921.0000028
AGTTTTT454.329304E-721.00000219
ACGAACT1100.021.0000027
ACGAACA900.021.0000027
CGTTTTT454.329304E-721.00000219
GGGTTTT303.2854883E-421.018
GGTGACG353.6184807E-521.03
TTTTTTT3700.021.021
TGTTTTT504.6964487E-821.019
GAACGGG605.4205884E-1021.09
GAACGGC504.6964487E-821.09
AGGGTGA353.6184807E-521.01
GAACGAG303.2854883E-421.09
GTTTTTT1950.021.020
GAACCTG250.002972578421.09
GCTGTCT353.6184807E-521.020
AGTGTGA4600.021.01
CGAACTT250.002972578421.08
CGAACTG504.6964487E-821.08
Produced by FastQC (version 0.11.2)