FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_76.3510000005dff3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_76.3510000005dff3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8159
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC780.9559995097438412No Hit
AGAGGACGAGCTGTCTCTTATACACAT440.539281774727295No Hit
AGAGGACGAGATCTGTCTCTTCTACAC260.31866650324794704No Hit
AGAGGACGAGCTGTCTCTTATCCACAT180.22061527147934795No Hit
AGAGGACGAGATGCTGTCTCTTATACA110.13482044368182375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGG353.925526E-521.00000210
CAATTTT250.00310400521.00000218
CATTTTT250.00310400521.00000219
CCGTTTT250.00310400521.00000218
CGGACGA556.159098E-921.0000023
GCGGACG556.159098E-921.0000022
AGCGGAC556.159098E-921.0000021
TATTTTT250.00310400521.00000219
GGTGGTT250.00310400521.00000216
GTGGTTT353.925526E-521.00000217
GCATTTT250.00310400521.00000218
GAGATGT709.094947E-1221.0000028
GAGATGG1400.021.0000028
GAGATGC556.159098E-921.0000028
GAGATGA353.925526E-521.0000028
GAGATCG556.159098E-921.0000028
GAGATAT556.159098E-921.0000028
GAGATAG709.094947E-1221.0000028
AGATAGG250.00310400521.0000029
GGACGAG8000.021.0000024
Produced by FastQC (version 0.11.2)