FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_73.3510000005dfa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_73.3510000005dfa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC90.23183925811437403No Hit
CTTGGTTCGTTGCTGTCTCTTCTACAC50.1287995878413189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG3800.021.0000026
AATTTTT303.0402135E-421.019
TCGGTTC250.00281337121.02
TGTTTTT403.4669465E-621.019
GGTTTTT654.0017767E-1121.019
GGTTCGT3750.021.04
CTTGGTT3100.021.01
TATTTTT453.6522033E-721.019
TCGTTGT703.6379788E-1221.07
TCGTTGG1300.021.07
TCGTTGC800.021.07
CTCGGTT250.00281337121.01
TTGGTTC3100.021.02
CGTTGTT303.0402135E-421.08
CGTTGGT303.0402135E-421.08
CGTTGGG703.6379788E-1221.08
CGTTGGC250.00281337121.08
CGTTGCG353.260411E-521.08
CGTTGCC303.0402135E-421.08
CGTTGAT353.260411E-521.08
Produced by FastQC (version 0.11.2)