FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_72.3510000005df89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_72.3510000005df89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9542
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC991.037518339970656No Hit
GTGTAGTGTGGCTGTCTCTTATACACA750.7859987424020122No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC320.3353594634248585No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC300.3143994969608049No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC280.2934395304967512No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC260.2724795640326975No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA260.2724795640326975No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC240.2515195975686439No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC200.20959966464053656No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT140.1467197652483756No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC120.12575979878432195No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC120.12575979878432195No Hit
GTGTAGTGTGGCTGTCTCTTATACCCA120.12575979878432195No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC110.1152798155522951No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC110.1152798155522951No Hit
GTGTAGTGTGGATACTGTCTCTTATAC110.1152798155522951No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC110.1152798155522951No Hit
GTGTAGTGTGGCTGTCTCTTCTACACA100.10479983232026828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTG9050.021.0000024
CATTTTT404.541631E-621.00000219
GTGGAGT800.021.0000028
TTGTTTT250.003142455421.018
GTGTTTT303.552343E-421.018
GTGTCGT455.1292955E-721.01
AATTTTT354.017284E-521.019
GTCGTGT455.1292955E-721.03
GTGTAGT7800.021.01
TGTTTTT1000.021.019
TTCTGTC354.017284E-521.021
CGTAGTG303.552343E-421.02
TCGTGTG455.1292955E-721.04
TGGAGTG303.552343E-421.09
TGGAGGT354.017284E-521.09
TGGAGGA354.017284E-521.09
TGGAGCG455.1292955E-721.09
TGGAGAG250.003142455421.09
GGGGGTT250.003142455421.016
AGTGTGG9000.021.05
Produced by FastQC (version 0.11.2)