FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_7.3510000005d767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_7.3510000005d767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42973
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1870.4351569590207805No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC580.1349684685732902No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT490.11402508551881414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC900.021.0000021
ACAGCAA900.021.0000023
GCGGGCG303.8492773E-421.00000211
TTACTTT303.8492773E-421.00000217
CGCCTTT303.8492773E-421.00000217
TACAGCA900.021.0000022
TGCGCGG609.877112E-1021.00000210
ACGTTTT456.0961065E-721.00000218
TGCGCCG303.8492773E-421.00000210
GGCGTTT456.0961065E-721.00000217
TGCCGGT303.8492773E-421.00000210
TGCCGAT303.8492773E-421.00000210
TAAGCAA303.8492773E-421.0000023
TGCGATG303.8492773E-421.00000210
GCGGCCG303.8492773E-421.00000211
GCGGTCG303.8492773E-421.00000211
CCGTTTT609.877112E-1021.00000218
ATGCGAC456.0961065E-721.0000029
TAGTTTT609.877112E-1021.00000218
ACATTTT303.8492773E-421.00000218
Produced by FastQC (version 0.11.2)