FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_69.3510000005df2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_69.3510000005df2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC220.27404085700049824No Hit
ACCTTGAACTGGGACTGTCTCTTATAC110.13702042850024912No Hit
ACCTTGAACTGGGCCTGTCTCTTATAC90.11210762331838565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT3950.021.1006321
GTCTGTC303.3700798E-421.1006320
CATTTTT303.3700798E-421.1006319
AGTTTTT303.3700798E-421.1006319
GGGTTTT250.003012471421.10062818
AATTTTT404.206826E-621.10062819
CCTGTCT353.767814E-521.10062820
TGTTTTT404.206826E-621.10062819
GTTTTTT1450.021.10062820
GACTGTC353.767814E-521.10062819
GCTTTTT404.206826E-621.10062819
GCCTGTC353.767814E-521.10062819
CTTTTTT800.021.10062820
CGTTTTT250.003012471421.10062819
ACTGGGT1050.020.9687528
ACTGGGG1350.020.9687528
CTGGGTG505.5784767E-820.968759
CTGGGTA250.00312585920.968759
AACTGGT1550.020.968757
AACTGGG3600.020.968757
Produced by FastQC (version 0.11.2)