FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_67.3510000005dee7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_67.3510000005dee7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC170.12128995433789953No Hit
TCAGAGCGCATTGGGGGGGGTTTTTTT150.10702054794520548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT608.276402E-1021.00000218
CATTGTG303.6727096E-421.0000029
CATTGGG751.8189894E-1221.0000029
CATTGCG303.6727096E-421.0000029
TTATTTT303.6727096E-421.00000218
CATTTTC303.6727096E-421.0000029
GAGTTTT303.6727096E-421.00000218
CATTATG303.6727096E-421.0000029
CATTATC303.6727096E-421.0000029
CAGAGCG11700.021.0000022
CATTAAA303.6727096E-421.0000029
CCATTTT303.6727096E-421.00000218
TAATTTT303.6727096E-421.00000218
GCGTTTT608.276402E-1021.00000218
GCATTTA751.8189894E-1221.0000028
GCATTAC608.276402E-1021.0000028
TGGTTTT404.8097627E-621.018
CATTGTT354.200157E-521.09
CATTGGC404.8097627E-621.09
CATTGGA250.003218032221.09
Produced by FastQC (version 0.11.2)