FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_63.3510000005de60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_63.3510000005de60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA1020.9225759768451519No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT310.28039073806078146No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC220.19898697539797394No Hit
TGGCATAGCTGACGGGCTGTCTCTTAT170.15376266280752532No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT170.15376266280752532No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA130.11758321273516643No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT130.11758321273516643No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT120.10853835021707671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT303.6044992E-421.018
GAGGCGG303.6044992E-421.011
TGGCATA10150.021.01
GTGTTTT455.2932955E-721.018
GGGTTTT1150.021.018
GAGCGGG303.6044992E-421.011
CTGAGGT303.6044992E-421.09
CTGAGGG1450.021.09
CTGAGGC800.021.09
CTGAGCG709.094947E-1221.09
CTGAGCA455.2932955E-721.09
AATTTTT303.6044992E-421.019
GCCTAGC303.6044992E-421.03
GTTCTGT303.6044992E-421.020
CGCTTTT303.6044992E-421.018
TTCTGTC354.096301E-521.021
GGATTTT354.096301E-521.018
TAGCTGA10900.021.06
GTTTTTT4200.021.020
CTGACGG404.6571076E-621.09
Produced by FastQC (version 0.11.2)