FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_62.3510000005de47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_62.3510000005de47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8160
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA891.0906862745098038No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC250.30637254901960786No Hit
ATGCTCTGTTGCGTCTGTCTCTTATAC180.22058823529411764No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC150.1838235294117647No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC140.1715686274509804No Hit
ATGCTCTGTTGCTGTCTCTTCTACACA110.13480392156862744No Hit
ATGCTCTGTTGCTCCTGTCTCTTATAC100.12254901960784313No Hit
ATGCTCTGTTGCTGTCTCTTATACCCA90.11029411764705882No Hit
ATGCTCTGTTGCAGCTGTCTCTTATAC90.11029411764705882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT250.00310400521.00000218
GGGTTTT505.5246346E-821.00000218
TTGCAGA250.00310400521.0000029
GGATTTT505.5246346E-821.00000218
CATTTTT353.925526E-521.00000219
ACTTTTT250.00310400521.00000219
GGGGGTT250.00310400521.00000216
TTCTTTT250.00310400521.00000218
TGCTTTT250.00310400521.00000218
GTCTTTT250.00310400521.00000218
AGGCTCT709.094947E-1221.0000021
GATTTTT709.094947E-1221.00000219
GGGATTT250.00310400521.00000217
AGGTTTT250.00310400521.00000218
TTGCGGG556.159098E-921.0000029
TTGCGGC505.5246346E-821.0000029
CGGTTTT353.925526E-521.00000218
TCTTTTT505.5246346E-821.00000219
GCTTTTT505.5246346E-821.00000219
ATTTTTT1400.021.00000220
Produced by FastQC (version 0.11.2)