FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_60.3510000005de03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_60.3510000005de03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1440.8449216687202956No Hit
GAATCCTTACGCCTGTCTCTTCTACAC600.35205069530012323No Hit
GAATCCTTACGCTGTCTCTTATACACA250.14668778970838467No Hit
GAATCCTTACGCCCTGTCTCTTATACA240.1408202781200493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT1000.021.047218
TAAGTTT250.00320412621.047217
GGATTTT506.3690095E-821.047218
CATTTTT250.00320412621.047219
ACTTTTT506.3690095E-821.047219
GGGATTT250.00320412621.047217
AGCGTTT250.00320412621.047217
TTGTTTT455.5368764E-721.04719718
TGGTTTT455.5368764E-721.04719718
CAGTTTT354.195699E-521.04719718
AATTTTT608.421921E-1021.04719719
ACGTTTT354.195699E-521.04719718
TTTCTGT303.6617392E-421.04719721
TTATTTT303.6617392E-421.04719718
GGTTTTT2500.021.04719719
GAGTTTT455.5368764E-721.04719718
CGGGTTT303.6617392E-421.04719717
TGCTTTT354.195699E-521.04719718
GTCTTTT354.195699E-521.04719718
CCTTTTT701.0913936E-1121.04719719
Produced by FastQC (version 0.11.2)