FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_53.3510000005dd2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_53.3510000005dd2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC130.5066250974279034No Hit
GAGCTACGTCTCTGTCTCTTATACACA70.2727981293842557No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC70.2727981293842557No Hit
GAGCTACGTCTAGAGGCTGTCTCTTAT60.23382696804364772No Hit
GAGCTACGTCTACCCTGTCTCTTATAC50.19485580670303976No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT50.19485580670303976No Hit
GAGCTACGTCTATGGGCTGTCTCTTAT50.19485580670303976No Hit
GAGCTACGTCTAGTCTGTCTCTTATAC40.1558846453624318No Hit
GAGCTACGTCTAGCCCTGTCTCTTATA40.1558846453624318No Hit
GAGCTACGTCTAGGCCTGTCTCTTATA40.1558846453624318No Hit
GAGCTACGTCTAGGGTCTGTCTCTTAT30.11691348402182386No Hit
GAGCTACGTCTATGGTCTGTCTCTTAT30.11691348402182386No Hit
GAGCTACGTCTACGGGCTGTCTCTTAT30.11691348402182386No Hit
GAGCTACGTCTAGACTGTCTCTTATAC30.11691348402182386No Hit
GAGCTACGTCTAGCCTGTCTCTTATAC30.11691348402182386No Hit
GAGCTACGTCTATGCTGTCTCTTATAC30.11691348402182386No Hit
GAGCTACGTCTAGGTCCTGTCTCTTAT30.11691348402182386No Hit
GAGCTACGTCTAGTTAGGTATTTTTTT30.11691348402182386No Hit
GAGCTACGTCTAAGCTGTCTCTTATAC30.11691348402182386No Hit
GAGCTACGTCTAGGAGCTGTCTCTTAT30.11691348402182386No Hit
GAGCTACGTCTCTGTCTCTTCTACACA30.11691348402182386No Hit
GAGCTACGTCTATACCTGTCTCTTATA30.11691348402182386No Hit
GAGCTACGTCTCTGTCTCTTATACCCC30.11691348402182386No Hit
GAGCTACGTCTATGGCTGTCTCTTATA30.11691348402182386No Hit
GAGCTACGTCTGTCTCTTATCCACCTC30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA2550.021.06
AGCTACG2300.021.02
GTTTTTT750.021.020
GAGCTAC2300.021.01
TACGTCT2550.021.05
CTACGTC2500.021.04
GTCTATG352.7053582E-521.08
GTCTATC302.6474395E-421.08
GTCTAAG302.6474395E-421.08
CGTCTAG602.3101165E-1021.07
CGTTTTT302.6474395E-421.019
TTTTTTT1700.020.99999821
GCTACGT2450.020.9999983
GTCTACG250.002551460920.9999988
ATTTTTT502.602792E-820.99999820
CGTCTAT950.020.9999987
CGTCTAC502.602792E-820.9999987
CGTCTAA502.602792E-820.9999987
CTTTTTT452.6855378E-720.99999820
Produced by FastQC (version 0.11.2)