FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_51.3510000005dce9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_51.3510000005dce9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC781.150781941575686No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC450.6639126586013573No Hit
GAGGAACAGAGCTGTCTCTTATACACA330.4868692829743287No Hit
GAGGAACAGAGTACCTGTCTCTTATAC80.11802891708468574No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA80.11802891708468574No Hit
GAGGAACAGAGTGGCTGTCTCTTATAC80.11802891708468574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT250.00304848921.00000218
GGGTTTT250.00304848921.00000218
TTTTTTT4000.021.00000221
AGTGGGG250.00304848921.00000210
GAGTTTG250.00304848921.0000029
GAGTCGG250.00304848921.0000029
GAGTCCG250.00304848921.0000029
CGGGTTT250.00304848921.00000217
AGAGTCC505.163929E-821.0000028
AGAGTAG555.677066E-921.0000028
AGAGTAC250.00304848921.0000028
AGTGAGG250.00304848921.00000210
AGTTTTT250.00304848921.00000219
GAGGCAC606.202754E-1021.01
GGCACAG606.202754E-1021.03
ACAGAGT6600.021.06
GAGTGGT303.404093E-421.09
GAGTGGG606.202754E-1021.09
GAGTGGC454.676749E-721.09
GAGTGAG303.404093E-421.09
Produced by FastQC (version 0.11.2)