FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_50.3510000005dccf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_50.3510000005dccf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGGCTGTCTCTTAT70.18031942297784648No Hit
GGTAGTGAGCTAGGGCCTGTCTCTTAT60.1545595054095827No Hit
GGTAGTGAGCTAGGGCTGTCTCTTATA50.1287995878413189No Hit
GGTAGTGAGCTAGAGCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTATAGCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTAGGTCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTAGGCTGTCTCTTATAC40.10303967027305513No Hit
GGTAGTGAGCTAAGGCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTAGGCCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTAGGGGGCTGTCTCTTA40.10303967027305513No Hit
GGTAGTGAGCTAGACCTGTCTCTTATA40.10303967027305513No Hit
GGTAGTGAGCTAGGTGCTTGTTTTTTT40.10303967027305513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTTT553.929017E-921.00000219
GAGCTAA750.021.0000027
GGGTTTT250.00281337121.018
TAGTGAG3150.021.03
GCTAGGG403.4669465E-621.09
GCTAGGA453.6522033E-721.09
AGCTAGT403.4669465E-621.08
AGCTAGC250.00281337121.08
AGCTAGA403.4669465E-621.08
AGCTACA353.260411E-521.08
AGCTAAG403.4669465E-621.08
TGTTTTT303.0402135E-421.019
GTATTTT303.0402135E-421.018
GTTTTTT1150.021.020
GAGCTAT353.260411E-521.07
GAGCTAG2050.021.07
GAGCTAC703.6379788E-1221.07
CTAGGGG250.00281337121.010
GTGAGCT3850.021.05
GCTACAG250.00281337121.09
Produced by FastQC (version 0.11.2)