FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_5.3510000005d724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_5.3510000005d724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31720
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1620.510718789407314No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC690.21752837326607818No Hit
GAGCTACGTCTCTGTCTCTTATACACA670.2112232030264817No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA470.14817150063051704No Hit
GAGCTACGTCTAGGGCCTGTCTCTTAT360.11349306431273644No Hit
GAGCTACGTCTAAGGGCTGTCTCTTAT330.10403530895334175No Hit
GAGCTACGTCTATGCTGTCTCTTATAC330.10403530895334175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT250.003281367721.03001420
GGTTTTT3300.020.99684719
ACTTTTT1450.020.99684719
TGCTACG1150.020.9968472
GTCTATA1650.020.9968478
GTCTAAC1450.020.9968478
GTGCTAC1150.020.9968471
TCCTTTT250.003311656420.99684518
ATCTTTT303.8224048E-420.99684518
TCTAGTG850.020.9968459
TCTAGTC751.8189894E-1220.9968459
TCTAGTA558.196366E-920.9968459
CTATAGC507.0080205E-820.99684510
TCGATTT250.003311656420.99684517
TCTAGGG1350.020.9968459
TCTAGGC1000.020.9968459
TCTAGGA651.1277734E-1020.9968459
TCTAGCT609.604264E-1020.9968459
TCTAGCG850.020.9968459
TCTAGCC507.0080205E-820.9968459
Produced by FastQC (version 0.11.2)