FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_44.3510000005dbf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_44.3510000005dbf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29681
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGC250.00330376321.00000210
AGCCACT27200.021.0000023
CTGGGGG701.2732926E-1121.00000210
CTGGGGC354.4115965E-521.00000210
CTGGGGA354.4115965E-521.00000210
TCTGGTG701.2732926E-1121.0000029
TCTGGTC354.4115965E-521.0000029
CTGGGAC250.00330376321.00000210
TAAGCCA354.4115965E-521.0000021
TCTGGGT1000.021.0000029
TCTGGGA850.021.0000029
TCTGGCT701.2732926E-1121.0000029
TCTGGCA701.2732926E-1121.0000029
TCTGGAT506.95818E-821.0000029
TCTGGAA250.00330376321.0000029
TGATTTT558.127245E-921.00000218
GCGGTTT250.00330376321.00000217
GGATTTT506.95818E-821.00000218
TAGCCAC25000.021.0000022
CTGGCGA250.00330376321.00000210
Produced by FastQC (version 0.11.2)