FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_42.3510000005dbc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_42.3510000005dbc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18033
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT354.288178E-521.00000218
ACTGCGG557.599738E-921.0000029
ACTGTGT250.003253930521.0000029
ACTGCCA250.003253930521.0000029
GTGTTTT506.580012E-821.00000218
CAGTTTT250.003253930521.00000218
CTGAGGG354.288178E-521.00000210
AAATTTT354.288178E-521.00000218
ACTGATT250.003253930521.0000029
ACTGATG557.599738E-921.0000029
GCGATTT250.003253930521.00000217
AATTTTT850.021.00000219
ACTGACG506.580012E-821.0000029
ACTGAAG354.288178E-521.0000029
CTGGGGT250.003253930521.00000210
CTGGGGG557.599738E-921.00000210
CTGGGGA354.288178E-521.00000210
GGTCTGT354.288178E-521.00000219
TGATTTT250.003253930521.00000218
GGATTTT557.599738E-921.00000218
Produced by FastQC (version 0.11.2)