FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_40.3510000005db70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_40.3510000005db70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32643
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1840.5636736819532518No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC710.21750451857978742No Hit
GTTGAGCTTGACGCTGTCTCTTATACA380.11641086909904115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT609.440555E-1021.02918621
ATTCTGT303.7859078E-421.02918620
GTTCTGT250.00328411121.02918420
GTTTCTG506.909431E-821.02918420
TGGTTTT900.020.99693318
GTAGAGC303.8255335E-420.9969331
ACCGGGG303.8255335E-420.99693311
GGGTTTT2100.020.99693318
GTTGAGC25550.020.9969331
GGTGAGC850.020.9969331
GCCTTTT303.8255335E-420.99693318
AGCTTTT456.011578E-720.99693318
CTGAGCT651.1277734E-1020.9969332
AGCTTGA29750.020.9969335
TGCGCTT2400.020.9969333
TGACCTG800.020.9969339
TGACCTA303.8255335E-420.9969339
AATTTTT1250.020.99693319
TGACCGG1150.020.9969339
TGACCGC609.622454E-1020.9969339
Produced by FastQC (version 0.11.2)