FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_39.3510000005db57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_39.3510000005db57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13885
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA370.2664746128916097No Hit
ACTTCGTCAGCTGTCTCTTATACACAT240.17284839755131437No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC170.12243428159884767No Hit
ACTTCGTCAGCTCCTGTCTCTTATACA160.11523226503420958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCA659.458745E-1121.0000028
CAGCTAG751.8189894E-1221.0000028
CAGCTAC751.8189894E-1221.0000028
GGTTTTT1500.021.00000219
ACTTCGT10900.021.0000021
TCAGCTA2600.021.0000027
AGTTTTT659.458745E-1121.00000219
CAGCTCG1150.021.08
CAGCTCC303.6699252E-421.08
CAGCTAT506.303708E-821.08
CAGCTAA608.258212E-1021.08
TGGTTTT303.6699252E-421.018
CGTCAGC12600.021.05
TGCATTT250.003216290821.017
GTGTTTT455.502534E-721.018
GGGTTTT800.021.018
AGCTCTT404.8034926E-621.09
AGCTTTG303.6699252E-421.09
AGCTCGG354.1959036E-521.09
AGCTCGC250.003216290821.09
Produced by FastQC (version 0.11.2)