FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_34.3510000005dab4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_34.3510000005dab4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1860.8723384297908263No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC690.3236094175030485No Hit
ACTCGTCTCGATGCTGTCTCTTATACA520.24387956101679015No Hit
ACTCGTCTCGATGGCTGTCTCTTATAC340.15945971297251665No Hit
ACTCGTCTCGATCCTGTCTCTTATACA270.1266297720664103No Hit
ACTCGTCTCGCTGTCTCTTATACACAT240.11255979739236469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG303.7617038E-421.00000211
GGGTTTT557.8052835E-921.00000218
CGATATT303.7617038E-421.0000029
CGATATG303.7617038E-421.0000029
AGCTTTT303.7617038E-421.00000218
CGATAGT557.8052835E-921.0000029
TCGATGA1900.021.0000028
TCGATAC950.021.0000028
TCGATAA900.021.0000028
GATACGG303.7617038E-421.00000210
TGATTTT303.7617038E-421.00000218
GTATTTT557.8052835E-921.00000218
GATCGGG303.7617038E-421.00000210
GATTGTG303.7617038E-421.00000210
GATGAGA303.7617038E-421.00000210
GCAGTTT303.7617038E-421.00000217
GTCTTTT557.8052835E-921.00000218
TCGTCTC18700.021.0000023
CGATGTT455.802631E-721.0000029
CGATGCA303.7617038E-421.0000029
Produced by FastQC (version 0.11.2)