FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_33.3510000005da9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_33.3510000005da9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25794
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA980.379933317825851No Hit
AACGGAATGGCTGCTGTCTCTTATACA700.2713809413041793No Hit
AACGGAATGGCTCCTGTCTCTTATACA550.2132278824532837No Hit
AACGGAATGGCTCTGTCTCTTATACAC550.2132278824532837No Hit
AACGGAATGGCTGTCTCTTATACACAT530.20547414127316427No Hit
AACGGAATGGCTACTGTCTCTTATACA330.1279367294719702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT651.09139364E-1021.00000218
GTGTTTT651.09139364E-1021.00000218
GCCTTTT303.7915562E-421.00000218
CAGTTTT354.3824668E-521.00000218
ATCTTTT303.7915562E-421.00000218
AGCTTTT250.003292053221.00000218
AAATTTT303.7915562E-421.00000218
ACGTTTT354.3824668E-521.00000218
AACGAAA354.3824668E-521.0000021
CTGGGCA303.7915562E-421.00000211
GCTGGTG250.003292053221.00000210
TGATTTT303.7915562E-421.00000218
GATGTTT303.7915562E-421.00000217
GCTGGGG701.2732926E-1121.00000210
GCTGGGC609.331416E-1021.00000210
GCTGGGA354.3824668E-521.00000210
ATGGTTT250.003292053221.00000217
AGGGTTT506.867958E-821.00000217
CCGTTTT303.7915562E-421.00000218
GGCTGGT609.331416E-1021.0000029
Produced by FastQC (version 0.11.2)