FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_31.3510000005da58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_31.3510000005da58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36261
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAGGGCTGTCTCTTATA390.10755356995118723No Hit
CAGACATCGCAAGCTGTCTCTTATACA370.10203800226138275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATC250.003317878821.00000210
TCCTTTT507.067683E-821.00000218
CAAGAGT250.003317878821.00000210
CAAGAGA250.003317878821.00000210
AATCTGT250.003317878821.00000219
GCAAGTT507.067683E-821.0000029
GCAAGTG850.021.0000029
GCAAGTC507.067683E-821.0000029
GCAAGGG2600.021.0000029
GCAAGGC850.021.0000029
GCAAGCT651.1459633E-1021.0000029
GCAAGCA250.003317878821.0000029
GAAATCG1300.021.0000023
TTTCTGT651.1459633E-1021.00000221
TATGTTT250.003317878821.00000217
CAACTGG250.003317878821.00000210
GGTTTTT4000.021.00000219
CAATTGG250.003317878821.00000210
CAACACT250.003317878821.00000210
GCAACTG507.067683E-821.0000029
Produced by FastQC (version 0.11.2)