FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_30.3510000005da4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_30.3510000005da4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27244
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1740.6386727352811628No Hit
AGTGTGACGAACCTGTCTCTTCTACAC610.22390251064454558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT250.003296796221.018
TGTGACG24750.021.03
AACGCGG405.097674E-621.010
AACGCGA250.003296796221.010
GTGTTTT1050.021.018
AACGCAG303.7992036E-421.010
AACGTAT250.003296796221.010
GGTGACG1050.021.03
AACAAGG354.3942564E-521.010
ATCTTTT303.7992036E-421.018
AGCTTTT354.3942564E-521.018
AACCGGG250.003296796221.010
AACCGGA250.003296796221.010
AACCGCT250.003296796221.010
AATTTTT701.2732926E-1121.019
CGGGGTT303.7992036E-421.016
GAGTGAC303.7992036E-421.02
AACTGGG354.3942564E-521.010
TTTCTGT303.7992036E-421.021
CGGGGGG651.09139364E-1021.012
Produced by FastQC (version 0.11.2)