FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_3.3510000005d6d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_3.3510000005d6d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62248
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC8311.3349826500449813No Hit
GAGGAACAGAGCTGTCTCTTATACACA2600.4176841023004755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG354.5134195E-521.00000213
GAGAAAC250.003344450821.0000021
CAGTTTT507.277049E-821.00000218
AAATTTT354.5134195E-521.00000218
TGCGTTT250.003344450821.00000217
ACAGAGC250.003344450821.0000026
GATATTT250.003344450821.00000217
TACAGAG507.277049E-821.0000025
GAGTGAC1000.021.0000029
GAGTGAA751.8189894E-1221.0000029
TAAGTTT250.003344450821.00000217
AGTAGGC354.5134195E-521.00000210
AAACAGA250.003344450821.0000024
AGTAGCG354.5134195E-521.00000210
GTCGTGG250.003344450821.00000211
GCTGGTT250.003344450821.00000216
CGCTTTT701.4551915E-1121.00000218
GTCCGAG250.003344450821.00000211
AGTGGTC354.5134195E-521.00000210
GCGCGTT250.003344450821.00000216
Produced by FastQC (version 0.11.2)