FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_29.3510000005da21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_29.3510000005da21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53829
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCACTGTCTCTTATACAC4890.9084322577049546No Hit
AAGGTCCAAGCCTGTCTCTTATACACA4600.8545579520332907No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG960.1783425291199911No Hit
AAGGTCCAAGCACCTGTCTCTTATACA570.1058908766649947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1000.021.01488518
GCCTTTT507.176641E-821.01488518
GATATTT250.003324916821.01488517
ACGTTTT507.176641E-821.01488518
GTCGTTT250.003324916821.01488517
TTTTCTG250.003324916821.01488521
TGTTTTT3650.021.01488519
CGGATTT250.003324916821.01488517
CGGGTTT250.003324916821.01488517
TCAGTTT250.003324916821.01488517
AGAGTTT250.003324916821.01488517
GACGTTT250.003324916821.01488517
ATTGTTT250.003324916821.01488517
TGCTTTT507.176641E-821.01488518
TATCTGT250.003324916821.01488519
GATCTTT250.003324916821.01488517
CTAATTT250.003324916821.01488517
CGTATTT250.003324916821.01488517
TCGTTTT558.44193E-921.01488518
GGGATTT250.003324916821.01488517
Produced by FastQC (version 0.11.2)