FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_27.3510000005d9ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_27.3510000005d9ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15202
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGAGCTGTCTCTTATACA210.13813971845809764No Hit
TTCGACCAACGACTGTCTCTTATACAC200.13156163662675965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACC557.363269E-921.0000021
GCCCAAC557.363269E-921.0000024
TTCGCCC557.363269E-921.0000021
TGACCAA557.363269E-921.0000023
CGCCCAA557.363269E-921.0000023
AACGAAA557.363269E-921.0000028
CATTTTT751.8189894E-1221.00000219
ACGAGTG557.363269E-921.0000029
CCCAACG557.363269E-921.0000025
TCGCCCA557.363269E-921.0000022
TTGACCA557.363269E-921.0000022
ATTTTTT2600.021.00000220
CAACGAC3000.021.0000027
TTGTTTT354.2312626E-521.018
GGGTTTT850.021.018
GCCTTTT303.6930686E-421.018
CAGTTTT354.2312626E-521.018
CCAACGA15050.021.06
AACGATT701.0913936E-1121.08
AACGATG1050.021.08
Produced by FastQC (version 0.11.2)