FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_26.3510000005d9c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_26.3510000005d9c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42314
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC5401.2761733705156686No Hit
ACTGTCTCTGTCTCTTATACACATCTC3810.9004112114193883No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1710.40412156732996174No Hit
ACTGTCTGTGTCTGTCTCTTATACACA550.12998062107104033No Hit
ACTGTCTGTGTCCCTGTCTCTTATACA500.11816420097367301No Hit
ACTGTCTGTCTCTTATACACATCTCCG470.11107434891525264No Hit
ACTGTCTCTGTCTCTTATACCCATCTC430.10162121283735878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCT558.381903E-921.0000021
GTGTCGA3000.021.0000028
AAATTTT609.858923E-1021.00000218
TGTCCGA609.858923E-1021.0000029
TGTCCCG609.858923E-1021.0000029
TGTCTCG558.381903E-921.0000029
TGTCCAT558.381903E-921.0000029
TGTCCAG303.847942E-421.0000029
GTCGCCA303.847942E-421.00000210
TGTCTAC303.847942E-421.0000029
TGTCTAA558.381903E-921.0000029
GTCGTAC303.847942E-421.00000210
GTCCGGT303.847942E-421.00000210
CGCTTTT558.381903E-921.00000218
TCGCGGG303.847942E-421.00000211
TGTCATT609.858923E-1021.0000029
TGTCATG609.858923E-1021.0000029
TGTCATC303.847942E-421.0000029
TGTCATA558.381903E-921.0000029
TGTCAGG751.8189894E-1221.0000029
Produced by FastQC (version 0.11.2)