FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_22.3510000005d93f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_22.3510000005d93f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46197
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCCCTGTCTCTTATACA1830.396129618806416No Hit
GTCAGAACTACCGCTGTCTCTTATACA1670.36149533519492605No Hit
GTCAGAACTACCTCTGTCTCTTATACA730.1580189189774228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCTG250.00329470221.04017320
ACTTTTT1300.021.01735319
TACTGTC250.003315552621.01735319
TCTTTTT751.8189894E-1221.01735319
AGTTTTT3000.021.01735319
AACTACC45750.020.9945856
ACCGGTG850.020.99458310
TGGTTTT1100.020.99458318
ACCGGTC405.239055E-620.99458310
ACCGGGG1600.020.99458310
ACCGGCG1050.020.99458310
ACCGGCA303.862031E-420.99458310
ACCGGAA303.862031E-420.99458310
GCCTTTT651.1823431E-1020.99458318
GCGATTT303.862031E-420.99458317
ACAGAAC1100.020.9945832
ATGATTT303.862031E-420.99458317
GGCGTTT609.968062E-1020.99458317
GACAGAA1100.020.9945831
CGGGGGG303.862031E-420.99458312
Produced by FastQC (version 0.11.2)