FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_20.3510000005d908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_20.3510000005d908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46392
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT4240.9139506811519227No Hit
AGTCTCCTACGTCTGTCTCTTATACAC3550.7652181410588033No Hit
AGTCTCCTACCTGTCTCTTATCCACAT1470.31686497672012415No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC1390.2996206242455596No Hit
AGTCTCCTACGCTGTCTCTTATACACA1050.22633212622866014No Hit
AGTCTCCTACGTGGCTGTCTCTTATAC540.11639937920331091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT303.830227E-421.02051721
AGCCTCC1050.020.9978451
GGGTTTT2100.020.99784518
GCCTCCT1050.020.9978452
ACGTCAG1050.020.9978459
TGTTTTT4050.020.99784519
CCTCCTA1050.020.9978453
TTGTTTT751.8189894E-1220.99784318
ATCCTAC558.445568E-920.9978434
TTCATTT250.003333724820.99784317
CGTCAGG354.485056E-520.99784310
GCCTTTT558.445568E-920.99784318
CAGTTTT651.1641532E-1020.99784318
GGCATTT250.003333724820.99784317
AGCTTTT558.445568E-920.99784318
CCTACCT354.485056E-520.9978436
TCGATTT250.003333724820.99784317
AAATTTT250.003333724820.99784318
AATTTTT1600.020.99784319
ACGTCTC405.232827E-620.9978439
Produced by FastQC (version 0.11.2)