FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_19.3510000005d8ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_19.3510000005d8ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50605
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC520.10275664460033594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAT303.8624342E-421.0000025
TTGTTGG303.8624342E-421.00000211
CATTGTC751.8189894E-1221.0000029
CATTGGT1200.021.0000029
CATTGGA1200.021.0000029
CATTGCA601.0004442E-921.0000029
GCCCATT303.8624342E-421.0000026
TGCGTTT303.8624342E-421.00000217
ATTAGGT303.8624342E-421.00000210
TATGTTT303.8624342E-421.00000217
GAAGTTT303.8624342E-421.00000217
ATTGGAA303.8624342E-421.00000210
CATTCTT601.0004442E-921.0000029
CATTCTC751.8189894E-1221.0000029
CATTTTG751.8189894E-1221.0000029
GAGCCCA303.8624342E-421.0000024
CTGATTT303.8624342E-421.00000217
ATAATTT303.8624342E-421.00000217
AACTTTT303.8624342E-421.00000218
GGTTATT303.8624342E-421.00000216
Produced by FastQC (version 0.11.2)