FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_18.3510000005d8c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_18.3510000005d8c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA1290.6050089109839603No Hit
GCATTGGATACCGGCTGTCTCTTATAC1040.4877591220335803No Hit
GCATTGGATACCCTGTCTCTTATACAC910.4267892317793828No Hit
GCATTGGATACCGTCTGTCTCTTATAC660.30953944282900286No Hit
GCATTGGATACCGCCTGTCTCTTATAC630.29546946815495734No Hit
GCATTGGATACCAGCTGTCTCTTATAC510.239189569458775No Hit
GCATTGGATACCCGCTGTCTCTTATAC460.215739611668699No Hit
GCATTGGATACCCCCTGTCTCTTATAC450.21104962011068382No Hit
GCATTGGATACCTGCTGTCTCTTATAC450.21104962011068382No Hit
GCATTGGATACCATCTGTCTCTTATAC380.17821967920457743No Hit
GCATTGGATACCGACTGTCTCTTATAC380.17821967920457743No Hit
GCATTGGATACCCTGTCTCTTCTACAC340.15945971297251665No Hit
GCATTGGATACCGCTGTCTCTTATACA270.1266297720664103No Hit
GCATTGGATACCAACTGTCTCTTATAC270.1266297720664103No Hit
GCATTGGATACCACCTGTCTCTTATAC220.1031798142763343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA303.7617038E-421.00000210
ACCGGAA303.7617038E-421.00000210
AGCTTTT303.7617038E-421.00000218
CATTGGA18600.021.0000022
AAATTTT557.8052835E-921.00000218
TTTCTGT303.7617038E-421.00000221
ACCGTAG303.7617038E-421.00000210
GAGTTTT557.8052835E-921.00000218
ACCGATG303.7617038E-421.00000210
ACTTTTT609.058567E-1021.00000219
ACCGAGT303.7617038E-421.00000210
CGGGTTT303.7617038E-421.00000217
TACCGCC455.802631E-721.0000029
TACCGCA303.7617038E-421.0000029
GTGATTT303.7617038E-421.00000217
TTTGGAT900.021.0000023
TACCCTG455.802631E-721.0000029
TACCCTA303.7617038E-421.0000029
TACCCGT303.7617038E-421.0000029
TACCCGC557.8052835E-921.0000029
Produced by FastQC (version 0.11.2)