FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_17.3510000005d8aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_17.3510000005d8aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18559
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC1380.7435745460423514No Hit
AACGTGCGTATGGGCTGTCTCTTATAC830.4472223718950374No Hit
AACGTGCGTATGGCTGTCTCTTATACA720.3879519370655747No Hit
AACGTGCGTATGCTGTCTCTTCTACAC550.29635217414731396No Hit
AACGTGCGTATCTGTCTCTTATACACA440.23708173931785118No Hit
AACGTGCGTATGCCTGTCTCTTATACA370.19936418988092033No Hit
AACGTGCGTATGGCCTGTCTCTTATAC360.19397596853278734No Hit
AACGTGCGTATGAGCTGTCTCTTATAC260.14009375505145752No Hit
AACGTGCGTATGTCTGTCTCTTATACA250.13470553370332453No Hit
AACGTGCGTATGACCTGTCTCTTATAC240.12931731235519156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA250.003257671821.00000210
ATGGGAG250.003257671821.00000210
GTATGCT950.021.0000028
GTATGCA506.608025E-821.0000028
GTATGAA1000.021.0000028
AAATTTT250.003257671821.00000218
GCGATTT250.003257671821.00000217
TGCGTAT18150.021.0000025
TATGCGC250.003257671821.0000029
TATGTGA250.003257671821.0000029
TATGCCT250.003257671821.0000029
TATGCAG250.003257671821.0000029
CGCTTTT250.003257671821.00000218
AGGGTTT250.003257671821.00000217
GTATTTT557.6397555E-921.00000218
ATGGCGG250.003257671821.00000210
ATGGCGA250.003257671821.00000210
CAATTTT250.003257671821.00000218
ATGAACG250.003257671821.00000210
TATGAGG506.608025E-821.0000029
Produced by FastQC (version 0.11.2)