FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_16.3510000005d880.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_16.3510000005d880.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64490
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC5150.7985734222360055No Hit
GAACACCATAGCGGCTGTCTCTTATAC3130.48534656535897036No Hit
GAACACCATAGCGCCTGTCTCTTATAC1830.2837649247945418No Hit
GAACACCATAGCGTCTGTCTCTTATAC1570.24344859668165605No Hit
GAACACCATAGCAGCTGTCTCTTATAC1470.2279423166382385No Hit
GAACACCATAGCTGCTGTCTCTTATAC1360.21088540859047913No Hit
GAACACCATAGCACCTGTCTCTTATAC1230.19072724453403628No Hit
GAACACCATAGCTGTCTCTTATACACA1160.17987284850364396No Hit
GAACACCATAGCCCCTGTCTCTTATAC1150.17832222049930221No Hit
GAACACCATAGCATCTGTCTCTTATAC1050.16281594045588463No Hit
GAACACCATAGCCGCTGTCTCTTATAC1030.15971468444720113No Hit
GAACACCATAGCGACTGTCTCTTATAC920.14265777639944177No Hit
GAACACCATAGCTCCTGTCTCTTATAC870.13490463637773298No Hit
GAACACCATAGCAACTGTCTCTTATAC730.11319584431694837No Hit
GAACACCATAGCCCTGTCTCTTATACA670.10389207629089782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG507.233393E-821.01475320
TTTCTGT950.021.01475121
ATTTCTG354.492244E-521.0147520
AGCCCGG250.003347184320.9984510
AGCCTGC250.003347184320.9984510
GCGGGGA354.519263E-520.9984511
TAGCGGT1450.020.998459
TAGCGGA1700.020.998459
TCCTTTT456.1994797E-720.9984518
GCGGGCG354.519263E-520.9984511
GCGGGAG354.519263E-520.9984511
TAGCGCT1250.020.998459
TAGCGCG1400.020.998459
TAGCGCA751.8189894E-1220.998459
GAGCGTT250.003347184320.9984516
TAGCGAG2450.020.998459
TAGCGAC1500.020.998459
GGGTCTG507.293056E-820.9984518
GGCATTT250.003347184320.9984517
AGCTTGC250.003347184320.9984510
Produced by FastQC (version 0.11.2)