FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_13.3510000005d816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_13.3510000005d816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34286
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA2160.6299947500437496No Hit
GACTCTATGGTCTGTCTCTTATACACA1740.5074957708685761No Hit
GACTCTATGGTGTCTGTCTCTTATACA1180.34416379863501134No Hit
GACTCTATGGTGCCTGTCTCTTATACA1170.3412471562736977No Hit
GACTCTATGGTGACTGTCTCTTATACA680.19833168056932857No Hit
GACTCTATGGTGCTGTCTCTTATACAC480.13999883334305546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT751.8189894E-1221.02046818
TCCTTTT354.404115E-521.02046818
TGCATTT303.8019952E-421.02046817
GGGTTTT1300.021.02046818
CAGTTTT455.9649574E-721.02046818
GGCATTT303.8019952E-421.02046817
ATCTTTT303.8019952E-421.02046818
TAAATTT250.003295406221.02046817
AAATTTT506.966002E-821.02046818
GGCGTTT250.003295406221.02046817
TTATTTT751.8189894E-1221.02046818
TGATTTT455.9649574E-721.02046818
GCGGTTT250.003295406221.02046817
AGGGTTT250.003295406221.02046817
GGATTTT950.021.02046818
GGTTTTT3250.021.02046819
CATTTTT1850.021.02046819
CCGTTTT303.8019952E-421.02046818
TAGTTTT354.404115E-521.02046818
GAGTTTT900.021.02046818
Produced by FastQC (version 0.11.2)