FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_10.3510000005d7c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_10.3510000005d7c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22967
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC3631.580528584490791No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC1480.6444028388557496No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1320.5747376670875605No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT620.2699525406017329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT557.887138E-921.00000218
TGCATTC751.8189894E-1221.0000028
TGCATCG1500.021.0000028
TGCATAT1300.021.0000028
TGCATAA751.8189894E-1221.0000028
TTCTGTC557.887138E-921.00000221
GTATTTT651.07320375E-1021.00000218
CCTTTTT751.8189894E-1221.00000219
GGCTTTT850.021.00000218
TATTTTT1700.021.00000219
GCATGTC557.887138E-921.0000029
GCATGGC751.8189894E-1221.0000029
GCATGGA557.887138E-921.0000029
GCATGCG850.021.0000029
CGGTTTT557.887138E-921.00000218
ATATTTT557.887138E-921.00000218
GCATCGG651.07320375E-1021.0000029
GCATTGG651.07320375E-1021.0000029
GCATAGG651.07320375E-1021.0000029
CGTTTCT250.003281219221.02
Produced by FastQC (version 0.11.2)