FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_1.3510000005d69f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_1.3510000005d69f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47447
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT1900.4004468143402112No Hit
GTGTCTGCTCCTGTCTCTTATCCACAT540.11381119986511265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1050.021.00000218
ATGGGGC354.484273E-521.00000211
GTGTTTT1050.021.00000218
CATGAGT354.484273E-521.00000210
CATGAGG354.484273E-521.00000210
CATGAGC405.232321E-621.00000210
CATGAAG751.8189894E-1221.00000210
AAATTTT651.1641532E-1021.00000218
TTTCTGT1050.021.00000221
TTTCTGC405.232321E-621.0000022
TTATTTT800.021.00000218
TGATTTT751.8189894E-1221.00000218
CTCTTTT405.232321E-621.00000218
TTCTGCT405.232321E-621.0000023
CCGTTTT405.232321E-621.00000218
CTGATTT354.484273E-521.00000217
CGGATTT354.484273E-521.00000217
TCTGCTC44000.021.0000024
GTCTTTT751.8189894E-1221.00000218
GGCTTTT1050.021.00000218
Produced by FastQC (version 0.11.2)