FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_93.3510000005d625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_93.3510000005d625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29941
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG10950.021.0000027
TCTAGAC1700.021.0000022
GCTAGGT900.021.0000029
GCTAGGC850.021.0000029
GCTAGCA455.9686136E-721.0000029
GCTAGAT455.9686136E-721.0000029
GCTAGAA455.9686136E-721.0000029
ATCTAGA1700.021.0000021
CGCTTTT455.9686136E-721.00000218
GAGTTTT900.021.00000218
GCTATGC455.9686136E-721.0000029
AGACGCT1700.021.0000025
TCATTTT455.9686136E-721.00000218
TAGACGC1700.021.0000024
CTCGACG27300.021.03
TGGTTTT701.2732926E-1121.018
TCCTTTT354.4132106E-521.018
GTGTTTT701.2732926E-1121.018
GGGTTTT950.021.018
CTATATG250.003304410521.010
Produced by FastQC (version 0.11.2)