FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_92.3510000005d5fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_92.3510000005d5fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57514
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG303.8507394E-421.01653721
ATTTTCT250.003326160121.01653720
CTTCTGT405.2286923E-621.01653520
CTCTGCA1850.020.9982628
CTCTGAG3750.020.9982628
AGCCACT52700.020.9982623
CTGGGTG850.020.99826210
TCTGGGG3750.020.9982629
TCTGGCG1700.020.9982629
TCTGGAG1700.020.9982629
TCTGGAC850.020.9982629
ACTCTGG24050.020.9982627
TGCTTTT850.020.99826218
TTAGACA1750.020.998261
CTCTGGT5450.020.998268
CTCTGGC5000.020.998268
TTGTTTT800.020.9982618
CTCTGCG4000.020.998268
CTCTGAT1950.020.998268
CTCTGAA1950.020.998268
Produced by FastQC (version 0.11.2)