FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_91.3510000005d5d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_91.3510000005d5d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29483
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA2180.7394091510361903No Hit
CTAGCTGGATGTCTGTCTCTTATACAC810.27473459281619916No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA450.15263032934233287No Hit
CTAGCTGGATCTGTCTCTTATACACAT330.11192890818437745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTTT1050.021.02721219
TTTCTGT751.8189894E-1221.02721221
GTTCTGT609.349606E-1021.02721220
TGATTTT609.349606E-1021.02721218
CGCTTTT609.349606E-1021.02721218
GGTTCTG455.8877595E-721.02721219
GGTTTCT303.7762086E-421.02721219
ACTTTTT900.021.02721219
CGGGTTT303.7762086E-421.02721217
TCGTTTT303.7762086E-421.02721218
GAATTTT303.7762086E-421.02721218
CTTATAC455.8877595E-721.02721218
GTGATTT303.7762086E-421.02721217
GGGGTTT455.8877595E-721.02721217
CGGTTTT609.349606E-1021.02721218
TCTTTTT900.021.02721219
ATCTGTC455.8877595E-721.02721220
ACGCTTT250.003278395221.0272117
GCCTTTT250.003278395221.0272118
AGCTTTT354.3661563E-521.0272118
Produced by FastQC (version 0.11.2)