FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_90.3510000005d5b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_90.3510000005d5b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36526
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCGA1300.021.0549471
TTCCAGA30300.021.0549471
TCCCAGA751.8189894E-1221.0549451
TTCAAGA250.003268243521.0549451
TTTCAGA800.021.0549451
TGCCAGA1500.021.0549451
TACCAGA900.021.0549451
ACTGCTA303.8373226E-420.9972619
ACTGTTG609.731593E-1020.9972619
ACTGTTA354.4523775E-520.9972619
ACTGTGT701.2732926E-1120.9972619
ACTGTGA609.731593E-1020.9972619
ACTGCCG303.8373226E-420.9972619
ACTGTCG456.051814E-720.9972619
ACTGCAA354.4523775E-520.9972619
ACTGTAA354.4523775E-520.9972619
GGGTCTG558.300049E-920.99726118
GGGTTTT850.020.99726118
CAGTTTT303.8373226E-420.99726118
CTGAGGG303.8373226E-420.99726110
Produced by FastQC (version 0.11.2)