FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_89.3510000005d590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_89.3510000005d590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC90.72No Hit
TCAACCGATAGGACCTGTCTCTTATAC20.16No Hit
TCAACCGATAGGAGATTGGGCTGTCTC20.16No Hit
TCAACCGATAGGGGGTTATGTTTTTTT20.16No Hit
TCAACCGATAGGCACTGTCTCTTATAC20.16No Hit
TCAACCGATAGGGGGTCGTGCTGTCTC20.16No Hit
TCAACCGATAGGACACAGCGTCTGTCT20.16No Hit
TCAACCGATAGGTATAGTAGCTGTCTC20.16No Hit
TCAACCGATAGGCCTCTTCGTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT1200.021.0000023
TTTTTTT603.092282E-1121.00000221
GATAGGA301.6740938E-421.0000027
CGATAGG1250.021.06
CAACCGA1150.021.02
GTTTTTT351.44566875E-521.020
TCAACCG1150.021.01
ACCGATA1250.021.04
CCGATAG1250.021.05
GATAGGT351.44566875E-521.07
GATAGGG351.44566875E-521.07
GATAGGC250.001853412620.9999987
Produced by FastQC (version 0.11.2)